Figure 2.

Determination of optimum concentration of KN-93 and Mito TEMPOL. (a) The Cell Counting Kit-8 (CCK-8) assay revealed that a concentration of 30 and 40 μM Mito TEMPOL impaired the proliferation capacity of BMSCs. ⁣^∗p < 0.05; ⁣^∗∗p < 0.01; ⁣^∗∗∗p < 0.001. (b) Live/dead cell staining demonstrated that Mito TEMPOL at concentrations ≤20 μM did not exhibit significant cytotoxicity toward BMSCs. (c) The ROS assay determined that the optimal concentration of Mito TEMPOL for inhibiting ROS was 20 μM. (d) Quantitative analysis of fluorescence intensity determined that the optimal concentration of Mito TEMPOL for ROS inhibition was 20 μM. ⁣^∗p < 0.05; ⁣^∗∗p < 0.01; ⁣^∗∗∗p < 0.001. (e) The CCK-8 assay revealed that 20 and 40 μM concentrations of KN-93 impaired the proliferation capacity of BMSCs at 48 and 72 h. ⁣^∗p < 0.05; ⁣^∗∗p < 0.01; ⁣^∗∗∗p < 0.001.