High-pressure inactivation kinetics of HorA at 200 MPa. The cells were grown at temperatures TG and were pressurized at temperatures TP. HorA activity was expressed relative to untreated cells. Shown are the means and standard deviations of three independent experiments. •, TG15/TP15; ○, TG30 + 5% ethanol/TP15; ▾, TG30/TP15; ▿, TG37/TP15; ▴, TG37/TP37; ▪, TG15/TP37. Control dead cells were prepared by pressure treatment at 800 MPa for 10 min.