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. 2025 Aug 22;16:1595. doi: 10.1007/s12672-025-03349-7

Table 1.

Biological attribution of Raman wave numbers

Raman wavenumber (cm− 1) Biomolecule assignment References
405 Glucose [26, 27]
525 DNA/RNA backbone vibrations, phosphodiester bond stretching or bending [28]
666 Guanine, Thymine (ring breathing modes in the DNA bases); tyrosine-G backbone in RNA [20]
780 Cytosine/uracil ring breathing (nucleotide) [29]
860 Tryptophan [30]
876 Hydroxyproline [21]
900 Saccharides [27]
934 Proline, valine [30]
1125 Amide III vibrations of proteins [31]
1247 Guanine, cytosine (NH2) [32,33]
1265 Unsaturated lipids [33]
1305 Amide III vibrations of proteins [31]
1440 CH2 bending mode of proteins and lipids [33]
1580 C-C stretching [34]
1603 C=C in-plane bending mode of phenylalanine and tyrosine [35]
1616 C=C stretching mode of tyrosine and tryptophan [35]
1655 Amide I vibrations of proteins [31]