TABLE 4.
Phenotypic characterization of the E. faecium strains studieda
| Characteristic | % of strains showing characteristic in genomic group:
|
|
|---|---|---|
| I (n = 62) | II (n = 16) | |
| Acetoin production | 97 | 94 |
| Alanine-phenylalanine-proline arylamidase | 2 | 0 |
| Pyroglutamic acid arylamidase | 98 | 100 |
| Glycyl-tryptophan arylamidase | 59 | 50 |
| α-Galactosidase | 34 | 63 |
| β-Galactosidase | 10 | 0 |
| β-Galactosidaseb | 84 | 81 |
| β-Glucosidase | 82 | 100 |
| N-Acetyl-β-glucosaminidase | 49 | 69 |
| Hydrolysis of hippurate | 51 | 44 |
| β-Mannosidase | 75 | 87 |
| Acidification of: | ||
| l-Arabinose | 92 | 81 |
| d-Arabitol | 2 | 6 |
| Cyclodextrin | 75 | 100 |
| Methyl-β-d-glucopyranoside | 80 | 81 |
| Glycogen | 0 | 19 |
| Lactose | 98 | 88 |
| Mannitol | 80 | 100 |
| Melezitose | 0 | 19 |
| Melibiose | 28 | 60 |
| Raffinose | 2 | 31 |
| Saccharose | 62 | 88 |
| Sorbitol | 0 | 19 |
| Tagatose | 13 | 38 |
| Trehalose | 97 | 100 |
All strains were positive for the arginine dihydrolase enzyme activity and acidification of maltose and ribose. None of the strains showed β-glucuronidase, alkaline phosphatase, or urease enzyme activity or acidified pullulane.
Measured by addition of Fast Blue BB reagent.