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. 2025 Jun 16;12(9):uhaf155. doi: 10.1093/hr/uhaf155

Figure 3.

Figure 3

Functional validation of OfTPS6 and OfTPS7 in vivo and in vitro.  A Aroma compound analysis of tobacco leaves of control, OfTPS6 and OfTPS7 overexpressed groups. B Extracellular enzyme function of OfTPS6 and OfTPS7 (GPP as substrate), 1: β-Myrcene; 2: D-limonene; 3: α-Pinene; 4: β-Ocimene; 5: Linalool. C Expressions of OfTPS6 and OfTPS7 in O. fragrans by RT-qPCR, Control: flowers were injected with empty vector, OE: flowers were injected with OfTPS6 and OfTPS7 overexpression vectors, respectively. D Linalool contents of O. fragrans flowers in control, overexpressed OfTPS6, OfTPS7 groups. E  OfTPS6 and OfTPS7 expression levels in O. fragrans flowers in control and silencing groups. F Linalool contents of O. fragrans flowers in control, silenced OfTPS6, OfTPS7 groups. All experiments were performed three times, each containing three biological replicates. The error line represents the average ± SD, and the asterisk represents the significant difference analysis compared with the control group, evaluated by one-way ANOVA. *P < 0.05, **P < 0.01, ***P < 0.001