Figure 6.

Expression profiles, sequence characterization and subcellular localization of OfWRKY33. A Partial sequence alignment between OfWRKY33 and AtWRKYs showing the conserved domain B. Evolutionary relationships among WRKY proteins from O. fragrans and other species were analyzed. The phylogenetic tree was generated using the maximum likelihood approach in MEGAX software. TPS protein information are available in Supplementary Table S5. C  OfWRKY33 subcellular localization, YFP fluorescence indicated the location of each fusion protein , the location of nucleus was determined by nuclear localization marker . D Expression patterns of OfWRKY33 in different tissue parts and flowering stages of 'BBYG'. E Expression levels of OfWRKY33 in the 20 cultivars. The 'Merged' column displayed the combined fluorescence signals from all channels. Bars = 100 μm. All experiments were performed three times, each containing three biological replicates. The error line represents the average ± SD, and the asterisk represents the significant difference analysis compared with the control group, evaluated by one-way ANOVA ^*P < 0.05, ^**P < 0.01, ^***P < 0.001