Figure 8.

OfWRKY33 binds directly to the W-box in the OfTPS7 promoter sequence. A Schematic diagram of dual luciferase carrier, the effector vector OfWRKY33 was constructed on the pGreen-62-SK vector driven by CaMV35S, and the reporter vector OfTPS7 promoter was constructed on the pGreen II-0800 LUC vector to drive the expression of LUC. B The transient fluorescence activity of tobacco indicates that OfWRKY33 has transcriptional activation activity and can promote the expression of OfTPS7.  C Relative fluorescence activity of OfWRKY33 transcriptional activity. D Yeast single heterozygous display of interaction between OfWRKY33 and OfTPS7 promoter. E OfWRKY33 binds directly to the OfTPS7 promoter, EMSA probe, Mut represents mutation probe, and mutation sites are represented by black dashed boxes, with " + " and "-" indicating the addition or absence of probes or proteins; 50× represents 50-fold unlabled cold probe, 100 × represents 100-fold unlabled cold probe, 1 × represents 1-fold unlabled mutant probe, 2 × represents 2-fold unlabled mutant probe, arrow indicating protein DNA complex or free probe position. All experiments were performed three times, each containing three biological replicates. The error line represents the average ± SD, and the asterisk represents the significant difference analysis compared with the control group, as determined by the Student’s t-test. ^*P < 0.05