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. 2025 Jun 18;12(9):uhaf148. doi: 10.1093/hr/uhaf148

Figure 4.

Figure 4

Downregulation of CmOFP6-19b resulted in reduced melon fruit size. Expression level of CmOFP6-19b in different melon cultivars. (A) Mature fruit phenotype of WT and CmOFP6-19b-RNAi lines. Bar = 5 cm. (B) Expression level of CmOFP6-19b in WT and RNAi lines (**P < 0.01, Student’s t-test). (C–F) Mature fruit longitudinal diameter, transverse diameter, weight and fruit shape index of WT, and RNAi lines, respectively (n = 20, **P < 0.01, Student’s t-test). (G) Longitudinal sections of WT and CmOFP6-19b-RNAi5 fruits at 10 DAP. Bar = 200 μm. (H, I) Cell number and cell area (n = 10) displayed in Panel G (**P < 0.01, Student’s t-test). (J) Expression of cell division- and cell expansion-related genes in CmOFP6-19b-RNAi5 fruit. (K) Fruit shape observation of different melon cultivars, B6, B7, E1, E6, B5, and E5. Bar = 10 cm. (L) Statistical analysis of fruit longitudinal diameter in different melon cultivars. (M) Expression level of CmOFP6-19b in different melon cultivars. Significance analysis was performed using one-way ANOVA. (N) Pearson correlation analysis showed negative correlation between the CmOFP6-19b expression level and the melon fruit longitudinal diameter.