Figure 5. SRSF1 contributes to PRMT5-mediated alternative splicing.

(A) Western blot analysis of MKL-1 cells treated with suboptimal (1.37 nM) or optimal (12 nM) JNJ-64619178, showing rescue of Tip60α exon inclusion by doxycycline-induced HA-SRSF1 expression. Bar graphs summarize quantification of full-length Tip60α levels normalized to housekeeping proteins from three independent experiments (n = 3, mean ± SD). Statistical significance was determined by a paired two-tailed t test. (B) MKL-1 cells with Dox-inducible EP400 shRNA were treated with JNJ-64619178 and Dox, then analyzed by Western blotting using the indicated antibodies. (C) MKL-1 cells expressing Dox-inducible HA-tagged SRSF1 were treated with serially diluted JNJ-64619178 for 2 d. Whole-cell lysates were immunoprecipitated with a YTHDC1 antibody and analyzed by Western blot using HA (SRSF1) and YTHDC1 antibodies.

Source data are available for this figure.