Figure S2. Correlation between PacBio full-length isoform sequencing and Illumina short-read sequencing using gene-level aggregated datasets.

(A) Venn diagram showing the overlap of up-regulated genes between Illumina short-read and PacBio full-length isoform datasets. The red circle represents genes in the short-read data, and the blue circle represents genes in the full-length isoform data. Overlapping areas indicate genes that are up- or down-regulated in both platforms. (B) Venn diagram depicting the overlap of down-regulated genes between short-read and full-length isoform datasets. (C) Scatter plot showing the Pearson correlation (r = 0.711) of gene expression levels between short-read and full-length isoform datasets in the treatment condition. Each point represents a gene, with expression values in the short-read dataset on the x-axis and the full-length isoform dataset on the y-axis (both log-transformed and normalized). (D) Scatter plot showing the Pearson correlation (r = 0.671) of gene expression levels between short-read and full-length isoform datasets in the control condition. Note: for this analysis, differential expression was assessed using a P-value threshold of 0.05 with no fold-change cutoff applied.