Table 2.
Antibody details for immunoblot analysis.
| Antibody | Host species | Dilution | Vendor | Catalog number |
|---|---|---|---|---|
| EP400 | Rabbit | 1:5,000 | Bethyl Laboratories | A300-541A |
| PRMT5 | Rabbit | 1:5,000 | Cell Signaling Technology | 79998 |
| HA | Mouse | 1:5,000 | Cell Signaling Technology | 2367 |
| SDMA | Rabbit | 1:5,000 | Cell Signaling Technology | 13222 |
| ADMA | Rabbit | 1:5,000 | Cell Signaling Technology | 13522 |
| MMA | Rabbit | 1:5,000 | Cell Signaling Technology | 8015 |
| GAPDH | Rabbit | 1:5,000 | Cell Signaling Technology | 5174 |
| γH2Ax | Rabbit | 1:5,000 | Cell Signaling Technology | 2577 |
| YTHDC1 | Rabbit | 1:5,000 | Cell Signaling Technology | 77422 |
| H4K5ac | Rabbit | 1:5,000 | Cell Signaling Technology | 8647 |
| H4K12ac | Rabbit | 1:5,000 | Cell Signaling Technology | 13944 |
| H4K16ac | Rabbit | 1:5,000 | Cell Signaling Technology | 13534 |
| H2A.Zac | Rabbit | 1:5,000 | Cell Signaling Technology | 75336 |
| H3 | Rabbit | 1:5,000 | Cell Signaling Technology | 4620 |
| Anti-rabbit IgG, HRP-conjugated | Goat | 1:5,000 | Cell Signaling Technology | 7074 |
| Anti-mouse IgG, HRP-conjugated | Horse | 1:5,000 | Cell Signaling Technology | 7076 |
| Tip60 (C-7) | Mouse | 1:5,000 | Santa Cruz Biotechnology | sc-166323 |
| MAX (C-17) | Rabbit | 1:5,000 | Santa Cruz Biotechnology | sc-197 |
| Vinculin | Mouse | 1:30000 | MilliporeSigma | V9131 |
| ASYM24 | Rabbit | 1:5,000 | MilliporeSigma | 07-414 |
| ASYM25 | Rabbit | 1:5,000 | MilliporeSigma | 09-814 |
List of antibodies used in immunoblot experiments, with host species, working dilution, vendor, and catalog information.