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. 2025 Apr 11;46(9):2468–2481. doi: 10.1038/s41401-025-01546-2

Fig. 2. JOSD2 deletion aggravates renal tubule injury and inflammation induced by IRI.

Fig. 2

a Schematic diagram depicting the procedure of IRI-induced AKI mice. b, c Representative immunoblots and relative mRNA levels of JOSD2 in renal cortex from wild-type control and IRI-induced AKI mice. (n  =  6; P values were determined by two-tailed unpaired t test and data are presented as mean ± SD; ***P < 0.001). d, e The levels of serum creatinine and urea nitrogen were analyzed in mice. f Representative images of H&E and PAS in mice. Scale bar: 50 μm. g Tubular injury of the kidneys quantified by tubular injury scores obtained from the sections. h Representative IF images of KIM-1 expression from mice. Scale bar, 50 μm. i, j Real-time qPCR showing mRNA levels of Il6 and Tnfα in kidney tissues of each group. (n  =  6; For (d, e, g, i, j), P values were determined by one-way ANOVA with Bonferroni’s correction and data are presented as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001).