Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalogue number |
|---|---|---|
| Experimental models | ||
| E. coli Top10 | Laboratory strain collection | F– mcrA DE(mrr-hsdRMS-mcrBC) φ80lacZ DE(M15) DE(lacX)74 recA1 araD139 DE(ara-leu)7697 galU galK λ– rpsL(StrR) endA1 nupG |
| E. coli BL21 (DE3) | Laboratory strain collection | F– ompT hsdSB (rB–, mB–) gal dcm (DE3) |
| E. coli C43 (DE3) | Laboratory strain collection | F– ompT hsdSB (rB–, mB–) gal dcm (DE3) lacUV5p |
| E. coli BW25113 | Keio collection (Baba et al, 2006) | F– rrnB DElacZ4787 HsdR514 DE(araBAD)567 DE(rhaBAD)568 rph-1 |
| E. coli J42W07 | Keio collection (Baba et al, 2006) | BW25113 DE(clpP)::kan |
| E. coli JW0428 | Keio collection (Baba et al, 2006) | BW25113 DE(clpX)::kan |
| E. coli JW0866 | Keio collection (Baba et al, 2006) | BW25113 DE(sspB)::kan |
| Recombinant DNA | ||
| pBAD-6xHis-SUMO-ClpX | Klimecka et al, 2021 | |
| pET28a-6xHis-SUMO-ClpP | Klimecka et al, 2021 | |
| pET28a-6xHis-SUMO-SspB | Klimecka et al, 2021 | |
| pET28a-His-ZBD | This study | |
| pET28a-GroEL | This study | |
| pET28a-6xHis-SUMO-XB-GGS | This study | |
| pET28a-6xHis-SUMO-GGS-SBP | This study | |
| pET28a-6xHis-SUMO-XB-GGS-SBP | This study | |
| pBAD-His-TEV-cAbGFP | This study | |
| pBAD-6xHIS-TEV | Klimecka et al, 2021 | |
| pBAD-6xHis-TEV-eGFP | Addgene | Cat #54762 |
| pBAD-6xHis-TEV-eGFP-ssrA | Klimecka et al, 2021 | |
| pBAD-6xHis-TEV-eGFP-AANDENY | Klimecka et al, 2021 | |
| pBAD-6xHis-TEV-eGFP-2xAANDENY | Klimecka et al, 2021 | |
| pBAD-6xHis-TEV-eGFP-XB | This study | |
| pBAD-6xHis-TEV-eGFP-sXB | This study | |
| pBAD-6xHis-TEV-eGFP-IGF | This study | |
| pBAD-6xHis-TEV-eGFP-IGL | This study | |
| pBAD-myc | This study | |
| pBAD-myc-XB-GGS | This study | |
| pBAD-myc-GGS-SBP | This study | |
| pBAD-myc-XB-GGS-SBP | This study | |
| pBAD-myc-XB-GGSGGSGG-SBP | This study | |
| pBAD-myc-NRLLLTG | This study | |
| pBAD-myc-XB-GGS-NRLLLTG | This study | |
| pBAD-myc-XB-GGSGG-NRLLLTG | This study | |
| pBAD-myc-pyrrhocoricin | This study | |
| pBAD-myc-XB-GGS-pyrrhocoricin | This study | |
| pBAD-myc-XB-GGSGGSGG-pyrrhocoricin | This study | |
| Chemicals, enzymes and other reagents | ||
| Antibodies | ||
| Mouse anti-GroEL | Invitrogen | MA3-022 |
| Rabbit anti-enolase | Gift from Professor Ben Luisi | |
| Goat anti-rabbit Alexa Fluor 488 | Invitrogen | A11008 |
| Goat anti-mouse Alexa Fluor 647 | Invitrogen | A32728 |
| Oligonucleotides | ||
| PCR primers | Thermo Fisher Scientific; this study | Appendix Table S3 |
| Oligonucleotides | Thermo Fisher Scientific; this study | Appendix Table S3 |
| Peptides | ||
| GroTAC3 | KareBay Biochem; this study | Appendix Table S4 |
| Synthetic GroTAC1, GroTAC2, Anchor-Linker, Linker-Bait | GeneScript; this study | Appendix Table S4 |
| Software | ||
| Image Lab | BioRad | |
| Graphpad Prism 9 | Graphpad Software | |
| MaxQuant v. 1.6.17.0 | Tyanova et al, 2016a | |
| Perseus v.1.6.10.0 | Tyanova et al, 2016b | |
| Octet Analysis Studio | Sartorius | |
| cryoSPARC 4.4 | Structura Biotechnology Inc. | |
| OccuPy | Forsberg et al, 2023 | |
| ModelAngelo | Jamali et al, 2024 | |
| cg2all | Heo and Feig, 2024 | |
| Coot | Emsley et al, 2010 | |
| Phenix | Liebschner et al, 2019 | |
| Other | ||
| BacTiter-Glo™ Microbial Cell Viability Assay | Promega GmbH | G8231 |
| Tecan Infinite M200 Pro | Tecan | |
| Octet R2 | Sartorius | |
| Octet NTA Biosensors | Sartorius | 18-5102 |
| ChemiDoc MP Universal Hood III | BioRad | |
| UltiMate 3000 nano-LC system | Thermo Fisher Scientific | |
| Q Exactive HF-X mass spectrometer | Thermo Fisher Scientific | |