Table 2.
Results for the isolates, whose biodegradation were verified by LC-HRMS.
| Isolate ID | Soil management | The closest relatives (% sequence identity, accession no.) | Solid-AMA (decrease of zone size in mm) | Liquid-AMA (Visible zone of inhibition in mm) | Biomass sorption assay | Average initial concentration of CLR (µg L− 1) ± SD (n = 3), LOQ (min-max) = 1.8–4.8 | Removal efficiency LC-HRMS (%) |
|---|---|---|---|---|---|---|---|
| BCCO_10_2490 | CMB | Nocardiopsis alba (99.79%, NR_026340.1) | ++ | 0 | NT | 1000 ± 60 | 100 |
| BCCO_10_2489 | CMB | Streptomyces intermedius/ koyangensis (99.72%, NR_041103.1/ CP031742.1) | ++ | 0 | NT | 1000 ± 0 | 100 |
| BCCO_10_2485 | CME | Streptomyces koyangensis (99.72%, NR_025662.1) | + | 0 | NT | 1000 ± 0 | 100 |
| BCCO_10_2486 | CME | Streptomyces anulatus/ pretensis/ praecox/ durocortorensis (100.00%, NR_112527.1/ NR_125619.1/ NR_112358.1/ MW582863.1) | +/- | 0 | not sorbed | 1100 ± 0 | 98.9 |
| BCCO_10_2496 | CME | Streptomyces resistomycificus/ griseochromogenes (100.00%, NR_042100.1/ NR_042102.1) | ++ | 0 | not sorbed | 1000 ± 60 | 100 |
| BCCO_10_2488 | CME | Streptomyces xiamenensis (99.51%, CP009922.3) | +++ | 0 | not sorbed | 1000 ± 0 | 100 |
| BCCO_10_2487 | CME | Streptomyces griseus (99.93%, EU048540.1) | ++ | 0 | not sorbed | 1100 ± 60 | 100 |
| BCCO_10_2492 | CMC | Streptomyces xiamenensis (99.20%, CP009922.3) | +++ | 0 | NT | 1000 ± 0 | 100 |
Experiments in solid-AMA were performed on MH agar in 28 °C for 3 days, while in liquid-AMA in MH broth in 28 °C for 5 days. Abbreviation: +/- decrease in ZD by < 3 mm, + decrease in ZD by 4–7 mm, ++ decrease in ZD by 8–14 mm, +++ decrease in ZD by > 15 mm; NT - not tested; CMW – soil irrigated with tap water, CME –soil irrigated with TWW, CMC – soil amended with composted biosolid and irrigate with TWW, CMB – soil amended with biosolid and irrigated with tap water.