Table 1.
SPR parameters
| α-Syn PFF | ||||
|---|---|---|---|---|
| Capture level (RU) | ka (1/M s) × 106 | kd (1/s) × 10−5 | KD (pM) | |
| SAR | 473 ± 2 | 4.2 ± 0.2 | 1.0a | <2.4 |
| 1E4 | 472 ± 3 | 4.0 ± 0.1 | 1.0a | <2.5 |
| α-Syn monomer | ||||
| Capture Level (RU) | ka (1/M s) × 106 | kd (1/s) × 10−5 | KD (pM) | |
| SAR | 410 ± 6 | 1.3 ± 0.1 | 0.53 ± 0.05 | 419 ± 60 |
| 1E4 | 537 ± 5 | 1.5 ± 0.1 | 0.68 ± 0.04 | 456 ± 20 |
Kinetic binding parameters of SAR446159 and 1E4 with α-Syn PFF or monomers. The off rate (kd) for the binding of the α-Syn PFF was below the limit of detection of the instrument and was fixed to the lowest limit for the dissociation time used in the experiment (1.0 × 10−5 1/s). The molarity of α-Syn aggregates was based on a particle size of 1 MDa. As a result, the binding affinities (KD) are estimates. The preferential binding of both molecules to α-Syn PFF over the monomer resulted in a five order of magnitude difference in affinity. All values are the average of n = 3.
akd below the limit of detection.