Table 2.
Mean and standard error (SEM) for the percentage of spermatozoa classified as intact acrosome and intact membrane (IAIM), damaged acrosome and intact membrane (DAIM), intact acrosome and damaged membrane (IADM), and damaged acrosome and damaged membrane (DADM), as well as overall acrosome damage (DA) and plasma membrane damage (DM) in mature (n = 20; 2–4 years) and senile (n = 20; ≥ 9 years) groups.
| Parameters | Mature | Senile | p-value |
|---|---|---|---|
| IAIM (%) | 77.7 ± 2.7 | 65.5 ± 2.7 | 0.003a |
| DAIM (%) | 4.20 ± 0.9 | 4.31 ± 0.6 | 0.883a |
| IADM (%) | 13.8 ± 2.5 | 25.3 ± 2.5 | 0.003a |
| DADM (%) | 4.9 ± 0.9 | 6.3 ± 0.9 | 0.296a |
| Damaged acrosome (DA) * (%) | 9.0 ± 1.2 | 10.4 ± 1.6 | 0.640b |
| Damaged membrane (DM) ** (%) | 8.4 ± 1.9 | 30.7 ± 3.5 | 0.003b |
aRepeated measures ANOVA.
bt-test.
* DA = DADM + DAIM.
** DM = DADM + IADM.
Spermatozoa were stained with PNA-Alexa Fluor™ 488 and propidium iodide (PI) fluorescent probes and analyzed using flow cytometry.