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. 2025 Aug 22;15:30976. doi: 10.1038/s41598-025-16410-w

Table 4.

Mean and standard error (SEM) number of canine spermatozoa bound to the zona pellucida (NBZ); comparison between mature and senile dogs, and post-thaw kinematic parameters in mature (n = 10), and senile (n = 10) groups.

Parameters Mature Senile P-valuea
NBZ 14.7 ± 2.0 7.3 ± 2.0 0.016
Total motility PT (%) 53.5 ± 2.6 20.8 ± 2.6  < 0.001
Progressive motility PT (%) 37.1 ± 1.9 8.0 ± 1.9  < 0.001
VAP PT (µm/s) 88.5 ± 2.6 61.7 ± 2.6  < 0.001
VSL PT (µm/s) 82.7 ± 1.2 51.6 ± 1.6  < 0.001
VCL PT (µm/s) 129.0 ± 3.9 115.0 ± 3.9 0.030
SLOW PT (%) 5.8 ± 0.7 8.4 ± 0.7 0.022
STATIC PT (%) 55.2 ± 4.9 73.8 ± 4.9 0.016

aRepeated measures ANOVA.

NBZ = mean number of spermatozoa bound to zona pellucida.

PT = post-thaw.

VAP = average path velocity.

VSL = straight line velocity.

VCL = curvilinear velocity.

SLOW = slowly motile spermatozoa.

STATIC = static sperm.