Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| Mouse LLC cells | ATCC | CRL-1642 |
| Human LK-2 | RIKEN BRC | RCB1970 |
| Human PC-9 | RIKEN BRC | RCB4455 |
| Human A549 | ATCC | CCL-185 |
| Human H1975 | ATCC | CRL-5908 |
| C57BL/6J mice | Kyudo | N/A |
| Recombinant DNA | ||
| Plasmid: PX459 (CRISPR) | Addgene | Plasmid #62988 |
| Plasmid: lentiCRISPR v2 (CRISPR) Puro | Derived from Addgene plasmid #83480 by replacing the blasticidin S-resistance gene with a puromycin-resistance gene | Plasmid # 83480 |
| pLVSIN-CMV vector | Takara | Cat#: 6183 |
| pLVSIN-CMV HA-YAP | This paper | N/A |
| pLVSIN-CMV EGFP | This paper | N/A |
| pLVSIN-CMV tdTomato | This paper | N/A |
| pLenti-P2A and pLenti-P2B | Cosmobio | Cat#: LV003 |
| Antibodies | ||
| Anti-YAP/TAZ antibody | Santa Cruz | Cat#: sc-101199; RRID: AB_1131430 |
| Anti-cytokeratin (CK; clone AE1/AE3) antibody | Agilent Technologies | Cat#: M3515 |
| Anti-GCH1 antibody | Thermo Fisher Scientific | Cat#: PA5-120098; RRID: AB_2913670 |
| Anti-YAP/TAZ antibody | Cell Signaling | Cat#: 93622 |
| Anti-HA-Tag antibody | Cell Signaling | Cat#: 2999 |
| Anti-β-actin antibody | Cell Signaling | Cat#: 12620 |
| Horseradish peroxidase (HRP)-linked mouse IgG | Cell Signaling | Cat#: 7076 |
| Horseradish peroxidase (HRP)-linked rabbit IgG | Cell Signaling | Cat#: 7074 |
| Oligonucleotides and other sequence-based reagents | ||
| CRISPR targeting sequence: human YAP: 5′-CATCAGATCGTGCACGTCCG-3′ | This paper | N/A |
| CRISPR targeting sequence: human TAZ: 5′-TGTCTAGGTCCTGCGTGACG-3′ | This paper | N/A |
| CRISPR targeting sequence: mouse Yap: 5′-GGCAGCTTGCGAAGCCGCAT-3′ | This paper | N/A |
| CRISPR targeting sequence: mouse Taz: 5′-GGATTGGCGCGAGTGCGAGC-3′ | This paper | N/A |
| CRISPR targeting sequence: mouse Gch1: 5′- GCTCGGAGAACCCATTGGTG-3′ | This paper | N/A |
| qPCR primers for mouse Gapdh: 5′-GCCTGGAGAAACCTGCCAAGTATG-3′ and 5′-GAGTGGGAGTTGCTGTTGAAGTCG-3′ | This paper | N/A |
| qPCR primers for mouse Ankrd1: 5′-CGATGAGTATAAACGGACGGCACTC-3’ and 5′-GGATGGCTGTGGATTCAAGCATATC-3′ | This paper | N/A |
| qPCR primers for mouse Gch1: 5′-AGCAAGTCCTTGGTCTCAGTAAAC-3′ and 5′-ACCGCAATCTGTTTGGTGAGGC-3′ | This paper | N/A |
| qPCR primers for mouse Tfrc: 5′-GAAGTCCAGTGTGGGAACAGGT-3′ and 5′-CAACCACTCAGTGGCACCAACA-3′ | This paper | N/A |
| qPCR primers for mouse Emp1: 5′-TCCCTGTCCTACGGCAATGAAG-3′ and 5′-CTGGAACACGAAGACCACAAGG-3′ | This paper | N/A |
| qPCR primers for mouse Acsl4: 5′-CCTTTGGCTCATGTGCTGGAAC-3′ and 5′-GCCATAAGTGTGGGTTTCAGTAC-3′ | This paper | N/A |
| qPCR primers for mouse Angptl4: 5′-CTGGACAGTGATTCAGAGACGC-3′ and 5′-GATGCTGTGCATCTTTTCCAGGC-3′ | This paper | N/A |
| Chemicals, enzymes and other reagents | ||
| (1S,3 R)-RSL3 | Selleck Chemicals | Cat#: S8155 |
| Erastin | Cayman | Cat#: 17754 |
| L-Buthionine-(S,R)-Sulfoximine | Cayman | Cat#: 14484 |
| Sulfasalazine | MedChemExpress | Cat#: HY-14655 |
| RPMI 1640 | MP Biomedicals | Cat#: 1646454 |
| Dialyzed Fetal Bovine Serum | Serana | Cat#: S-FBS-NL-065 |
| L-Methionine | Nacalai | Cat#: 13038-72 |
| L-Glutamine | FUJIFILM Wako | Cat#: 073-05391 |
| actinomycin D | FUJIFILM Wako | Cat#: 018-21264 |
| TNF-α | Abcam | Cat#: ab206641 |
| cycloheximide | FUJIFILM Wako | Cat#: 037-20991 |
| Z-VAD-FMK | Selleck Chemicals | Cat#: S7023 |
| Necrostatin-1 | Selleck Chemicals | Cat#: S8037 |
| Ferrostatin-1 | Cayman Chemical | Cat#: 17729 |
| (6 R)-5,6,7,8-tetrahydro-L-biopterin | Cayman Chemical | Cat#: 81880 |
| 7,8-dihydro-L-biopterin | Cayman Chemical | Cat#: 81882 |
| DAHP | Cayman Chemical | Cat#: 81260 |
| Lipofectamine 3000 transfection kit | Invitrogen | Cat#: L3000-08 |
| Puromycin | Thermo Fisher Scientific | Cat#: A1113803 |
| PolyJet | SignaGen | Cat#: SL100688 |
| Polybrene | Merck | Cat#: TR-1003-G |
| Propidium iodide | Dojindo | Cat#: 341-07881 |
| BODIPY 581/591 C11 | Thermo Fisher Scientific | Cat#: D3861 |
| Phos-tag (TM) Acrylamide AAL-107 | FUJIFILM Wako | Cat#: 304-93521 |
| Software | ||
| Prism 10 | GraphPad | https://www.graphpad.com/ |
| FlowJo | BD Biosciences | https://www.flowjo.com/solutions/flowjo/downloads |
| Adobe Illustrator | Adobe | https://www.adobe.com/products/illustrator.html |
| Adobe Photoshop | Adobe | https://www.adobe.com/products/illustrator.html |
| Other | ||
| Peroxidase Stain DAB Kit | Nacalai Tesque | Cat#: 25985-50 |
| 3-amino-9-ethylcarbazole | Nichirei | Cat#: 415182 |
| VECTASTAIN Elite ABC Rabbit IgG Kit | Vector Laboratories | Cat#: PK-6101 |
| TRIzol reagent | Invitrogen | Cat#: 15596026 |
| ReverTra Ace qPCR RT kit | TOYOBO | Cat#: FSQ-301 |
| SYBR qPCR Mix | TOYOBO | Cat#: QPX-201 |
| RNeasy Mini Kit | QIAGEN | Cat#: 74104 |
| NEBNext Poly(A) mRNA Magnetic Isolation Module | New England Biolabs | Cat#: E7490 |
| NEBNext Ultra II Directional RNA Library Prep Kit | New England Biolabs | Cat#: E7760 |
| RNA-Scope H2O2 & Protease Plus Reagents Kit | Advanced Cell Diagnostics | Cat#: 322330 |
| RNA-Scope 2.5 HD Duplex Detection Reagents Kit | Advanced Cell Diagnostics | Cat#: 322500 |
| RNA-Scope Probe Mm-GCH1-C2 | Advanced Cell Diagnostics | Cat#: 452601-C2 |
| RNA-Scope Negative Control Probe | Advanced Cell Diagnostics | Cat#: 310043 |
| Tn5 transposase | Illumina | Cat#: 20034197 |
| MinElute Reaction Cleanup Kit | QIAGEN | Cat#: 28206 |
| NEBNext High Fidelity 2 × PCR Master Mix | New England Biolabs | Cat#: M0541S |
| SPRIselect beads | Beckman Coulter | Cat#: B23319 |
| BCA Protein Assay Kit | FUJIFILM Wako | Cat#: 297-73101 |