Table 3.

List of primer sequences for homology arm and sgRNA target sequences employed in this study

Region	Homology Arm Primers	sgRNA target sequence
N1	F′–ACTGCTCCCTTGTCCTTGCTTCCAGGCCCGCAATGGGAGGTAAGCCCTT GCATTCG R′–GAACAGATCAACGGCAAAAGCCGAATTTGCTAGTTGCAGGGCATCAC CGCTTCCACTACCTGAACC	AGCCGAATTTGCTAGTTGCA
N2	F′–CTAACTGCTCCCTTGTCCTTGCTTCCAGGCCCGCAATGGGAGGTAAGC CCTTGCATTCG R′–ACGGCAAAAGCCGAATTTGCTAGTTGCAGGGCATCACCGCTTCCACTA CCTGAACC	AGTTGCAGGGCATCCATTG
C	F′–GAAACATTATTTTCTTTGGCAAATTCTGTTCTCCTGGAAGTGGCTCAGGT TCTGGA R-GAAAAGTCAGGGAGGACTTAACATGGGCTATGCCACTTACTTGGCCGATC GCATACAGAG	AACATGGGCTATGCCACTTA