Table 2.

ISSR and RAPD primers used for PCR amplification to evaluate genetic diversity in walnut genotypes distributed in inner Anatolia region Türkiye.

ISSR Primers Code	DNA Sequences (5’−3’)	Tm oC	Ta oC	PCR amplification
UBC-808	5′-AGAGAGAGAGAGAGAGC-3′	52	49	94 °C–3 min 94 °C–1 min 48–53 °C 1 min 72 °C–1 min 72 °C–10 min 35 cycles
UBC-810	5′-GAGAGAGAGAGAGAGAT-3′	50	47
UBC-811	5′-GAGAGAGAGAGAGAGAC-3′	53	50
UBC-826	5′-ACACACACACACACACC-3′	52	48
UBC-831	5′-CTCTCTCTCTCTCTCTT-3′	50	48
UBC-834	5′-AGAGAGAGAGAGAGAYT-3′	52	50
UBC-836	5′-AGAGAGAGAGAGAGAGYA-3′	52	50
UBC-873	5′-GACAGACAGACAGACA-3′	48	46
UBC-880	5′-GGAGAGGAGAGGAGA-3′	55	53
	RAPD Primers Code
OPA-2	5′-TGCCGAGCTG-3′	34	32	94 °C–3 min 94 °C–1 min 30–32 °C 1 min 72 °C–1 min 72 °C–10 min 35 cycles
OPA-5	5′-AGGGGTCTTG-3′	32	30
OPA-7	5′-GAAACGGGTG-3′	32	30
OPA-18	5′-AGGTGACCGT-3′	32	30
OPA-20	5′-GTTGCGATCC-3′	32	30

Tm = melting temperature, Ta = annealing temperature.