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. 2025 Aug 22;15:30955. doi: 10.1038/s41598-025-16887-5

Table 3.

Dynamic changes of mushroom substrate parameters during mycelial and fruiting body growth. All data were gathered prior to water stimulation.

Characteristics Time CK B1 B2 B3 PB1 PB2 PB3
pH 4d 6.77 ± 0.12 6.60 ± 0.11 6.78 ± 0.12 6.94 ± 0.12 6.77 ± 0.13 6.58 ± 0.12 7.02 ± 0.15
8d 6.51 ± 0.11 6.77 ± 0.13 6.45 ± 0.12 6.84 ± 0.11 6.65 ± 0.12 6.67 ± 0.11 6.76 ± 0.13
12d 6.37 ± 0.11 6.68 ± 0.11 6.54 ± 0.11 6.67 ± 0.10 6.41 ± 0.11 6.80 ± 0.13 6.83 ± 0.12
16d 6.00 ± 0.10 6.35 ± 0.09 6.64 ± 0.11 6.94 ± 0.14 6.30 ± 0.11 6.66 ± 0.12 6.68 ± 0.12
20d** 6.19 ± 0.10 6.46 ± 0.11 6.45 ± 0.12 6.58 ± 0.12 6.22 ± 0.12 6.32 ± 0.12 6.91 ± 0.13
2nd harvest* 4.85 ± 0.08 6.38 ± 0.12 6.52 ± 0.13 6.44 ± 0.11 6.19 ± 0.10 6.57 ± 0.11 6.50 ± 0.11
Moisture (%) 4d 60.31 ± 1.21 61.39 ± 1.23 60.43 ± 1.21 60.66 ± 1.21 61.50 ± 1.21 60.70 ± 1.21 61.50 ± 1.23
8d 63.96 ± 1.34 61.91 ± 1.24 61.93 ± 1.24 65.45 ± 1.31 62.77 ± 1.26 64.30 ± 1.29 65.24 ± 1.30
12d 66.92 ± 1.31 65.65 ± 1.31 62.47 ± 1.25 63.67 ± 1.27 65.82 ± 1.32 64.35 ± 1.29 64.84 ± 1.29
16d 68.69 ± 1.37 65.42 ± 1.32 64.19 ± 1.28 61.09 ± 1.22 62.41 ± 1.23 61.58 ± 1.23 60.83 ± 1.22
20d** 72.22 ± 1.44 65.10 ± 1.30 60.85 ± 1.22 61.34 ± 1.23 64.12 ± 1.28 62.50 ± 1.25 60.98 ± 1.22
2nd harvest* 51.73 ± 1.44 58.61 ± 1.17 59.92 ± 1.20 60.87 ± 1.22 63.51 ± 1.17 62.28 ± 1.15 61.44 ± 1.19
EC (dS/m) 4d 10.12 ± 0.51 15.32 ± 0.77 15.95 ± 0.80 16.82 ± 0.84 21.33 ± 1.07 21.43 ± 1.07 21.72 ± 1.09
8d 10.54 ± 0.53 15.44 ± 0.78 16.02 ± 0.82 17.12 ± 0.86 21.75 ± 1.09 21.56 ± 1.08 21.93 ± 1.11
12d 10.78 ± 0.54 15.89 ± 0.79 16.23 ± 0.81 17.33 ± 0.87 21.82 ± 1.11 21.89 ± 1.09 22.01 ± 1.08
16d 11.21 ± 0.56 16.12 ± 0.81 16.92 ± 0.85 17.54 ± 0.88 21.96 ± 1.10 22.06 ± 1.10 22.18 ± 1.12
20d** 12.31 ± 0.62 16.83 ± 0.84 17.81 ± 0.89 17.89 ± 0.89 22.15 ± 1.07 22.37 ± 1.12 22.41 ± 1.12
2nd harvest* 9.75 ± 0.49 14.61 ± 0.73 15.13 ± 0.76 16.57 ± 0.83 20.97 ± 1.05 21.29 ± 1.06 21.63 ± 1.08

* Owing to the initial inconsistency in the first harvest timing for each treatment, the subsequent harvest times differed as well, resulting in the final measurements being dependent on the actual harvest time for each treatment.

** After twenty days post-spawning, primordia emerged in certain treatments, requiring water for inducing regular fruiting, while others had to postpone. Consequently, the timing of water stimulation varied among treatments after the initial twenty days, rendering the collection of pH and moisture data unnecessary.