Reagents and tools table
| Reagent/resource | Reference or source | Identifier or catalog number |
|---|---|---|
| Experimental models | ||
| C. elegans wild isolate var Bristol | Caenorhabditis Genetics Center | N2 |
| wago-1(ok1074) I. deletion; presumed null | Caenorhabditis Genetics Center | RB1096 |
| wago-3(pk1673) I. deletion; presumed null | Caenorhabditis Genetics Center | NL5117 |
| wago-4(tm1019) II. deletion; presumed null | Barstead et al, 2012 | RFK22 |
| hrde-1(tm1200) III. deletion; presumed null | Caenorhabditis Genetics Center | YY538 |
| pei-1(ok1050) IV. deletion; presumed null | Caenorhabditis Genetics Center | RB1083 |
| pei-2(xf270) V. deletion; presumed null | Schreier et al, 2022 | RFK1342 |
| pgl-1(xf233[pgl-1::mTagRfp-t]) IV. | Schreier et al, 2022 | RFK1086 |
| ttTi5605 II; unc-119(ed3) III; oxEx1578[eft-3p::gfp + Cbr-unc-119(+)]. | Frøkjær-Jensen et al, 2012 | EG6699 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II. | This study | RFK1305 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; him-5(e1490) V. | This study | RFK1622 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV. | This study | RFK1527 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; wago-1(ok1074) I. | This study | RFK1513 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; wago-3(pk1673) I. | This study | RFK1520 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; wago-4(tm1019) II. | This study | RFK1515 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; hrde-1(tm1200) III. | This study | RFK1494 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; znfx-1(xf347) II. | This study | RFK1615 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV; him-5(e1490) V. | This study | RFK1405 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV; him-5(e1490) V; wago-1(ok1074) I. | This study | RFK1512 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV; him-5(e1490) V; wago-3(pk1673) I. | This study | RFK1470 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV; him-5(e1490) V; wago-4(tm1019) II. | This study | RFK1514 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV; him-5(e1490) V; hrde-1(tm1200) III. | This study | RFK1473 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV; him-5(e1490) V; znfx-1(xf347) II. | This study | RFK1619 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV; him-5(e1490) V; pei-1(ok1050) IV. | This study | RFK1471 |
| xfSi255[his-67p::gfp::his-67::tbb-2 3’UTR + Cbr-unc-119( + )] II; pgl-1(xf233[pgl-1::mTagRfp-t]) IV; him-5(e1490) V; pei-2(xf270) V. | This study | RFK1472 |
| Recombinant DNA | ||
| Plasmid—Induces Mos1 transposition by injection. | Addgene | pCFJ601 - #34874 |
| Plasmid—Negative selection, heat-shock inducible. | Addgene | pMA122 - #34873 |
| Plasmid—Visual extra-chromosomal array marker. Red, nervous system, cytosolic. | Addgene | pGH8 - #19359 |
| Plasmid—Visual extra-chromosomal array marker. Red, body wall muscle, cytosolic. | Addgene | pCFJ104 - #19328 |
| Plasmid—Visual extra-chromosomal array marker. Red, pharynx, cytosolic. | Addgene | pCFJ90 - #19327 |
| Plasmid—Multiple cloning site vector for MosSCI on locus ttTi5605 | Addgene | pCFJ350 - #34866 |
| Plasmid—gfp::SEC::3xflag parental vector for CRISPR/Cas9 insertions | Addgene | pDD282 - #66823 |
| Plasmid—[his-67p::gfp::his-67::tbb-2 3’UTR] insertion in pCFJ350 | This study | pRFK4197 |
| Plasmid—Expression of Cas9 and sgRNA(F + E) without protospacer | Schreier et al, 2022 | pRFK2411 |
| Plasmid—Expression of sgRNA(F + E) without protospacer | Schreier et al, 2022 | pRFK2412 |
| Plasmid—Expression of Cas9 and unc-58 sgRNA1(F + E) | Schreier et al, 2022 | pRFK2588 |
| Plasmid—Expression of znfx-1 sgRNA1(F + E) | This study | pRFK3358 |
| Plasmid—Expression of znfx-2 sgRNA1(F + E) | This study | pRFK3359 |
| Plasmid—Expression of znfx-3 sgRNA1(F + E) | This study | pRFK3360 |
| Plasmid—Expression of znfx-4 sgRNA1(F + E) | This study | pRFK3361 |
| Plasmid—Empty backbone for RNAi feeding experiments | Addgene | L4440 - #1654 |
| Plasmid—gfp sequence from pDD282 inserted in L4440 | This study | pRK4103 |
| Antibodies | ||
| Monoclonal mouse anti-GFP (B-2) | Santa Cruz Biotechnology | #sc-9996 |
| Monoclonal rabbit β-Actin (D6A8) | Cell Signaling Technology | #8457 |
| Anti-mouse IgG, HRP-linked antibody | Cell Signaling Technology | #7076 |
| Anti-rabbit IgG, HRP-linked antibody | Cell Signaling Technology | #7074 |
| Oligonucleotides and other sequence-based reagents | ||
| Protospacer sequence—ATC CAC GCA CAT GGT CAC TA | Arribere et al, 2014 | unc-58(e665) #1 |
| Protospacer sequence—CCA CCT CAA CGT GAG GTT GG | This study | znfx-1(xf347) #1 |
| Protospacer sequence—ATC CAT CGC GAT GCC CAT GG | This study | znfx-1(xf347) #2 |
| Protospacer sequence—TAA TCT CAC CAA TGC TTC GG | This study | znfx-1(xf347) #3 |
| Protospacer sequence—GAC ATC AGA TCG AAT GTT GG | This study | znfx-1(xf347) #4 |
| attcttctcctttactcat | LGC Biosearch Technologies | gfp smFISH probe #1 |
| gaggattgggacaactcca | LGC Biosearch Technologies | gfp smFISH probe #2 |
| cgttgacgtctccgtcgag | LGC Biosearch Technologies | gfp smFISH probe #3 |
| ccggagacggagaacttgt | LGC Biosearch Technologies | gfp smFISH probe #4 |
| gtgagctttccgtaggtgg | LGC Biosearch Technologies | gfp smFISH probe #5 |
| gtggtgcagatgaacttga | LGC Biosearch Technologies | gfp smFISH probe #6 |
| catgggactgggagctttc | LGC Biosearch Technologies | gfp smFISH probe #7 |
| aaggtggtgacgagggttg | LGC Biosearch Technologies | gfp smFISH probe #8 |
| agaagcattggactccgta | LGC Biosearch Technologies | gfp smFISH probe #9 |
| cttcatgtggtctgggtaa | LGC Biosearch Technologies | gfp smFISH probe #10 |
| ttgtagagctcgtccattc | LGC Biosearch Technologies | gfp smFISH probe #11 |
| ggcggacttgaagaagtcg | LGC Biosearch Technologies | gfp smFISH probe #12 |
| tcttggacgtatccctctg | LGC Biosearch Technologies | gfp smFISH probe #13 |
| cgtccttgaagaagatggt | LGC Biosearch Technologies | gfp smFISH probe #14 |
| gcacgggtcttgtagtttc | LGC Biosearch Technologies | gfp smFISH probe #15 |
| tgtctccctcgaacttgac | LGC Biosearch Technologies | gfp smFISH probe #16 |
| gctcgatacggttgacgag | LGC Biosearch Technologies | gfp smFISH probe #17 |
| tcctccttgaagtcgattc | LGC Biosearch Technologies | gfp smFISH probe #18 |
| ttgtgtccgaggatgtttc | LGC Biosearch Technologies | gfp smFISH probe #19 |
| gagttgtagttgtactcga | LGC Biosearch Technologies | gfp smFISH probe #20 |
| gccatgatgtagacgttgt | LGC Biosearch Technologies | gfp smFISH probe #21 |
| attccgttcttttgcttgt | LGC Biosearch Technologies | gfp smFISH probe #22 |
| cggatcttgaagttgacct | LGC Biosearch Technologies | gfp smFISH probe #23 |
| gatccgtcctcgatgttgt | LGC Biosearch Technologies | gfp smFISH probe #24 |
| tagtggtcggcgagttgga | LGC Biosearch Technologies | gfp smFISH probe #25 |
| ccgattggggtgttttgtt | LGC Biosearch Technologies | gfp smFISH probe #26 |
| ttgtctgggaggaggactg | LGC Biosearch Technologies | gfp smFISH probe #27 |
| gattgggtggagaggtagt | LGC Biosearch Technologies | gfp smFISH probe #28 |
| gtttgggtccttggagagg | LGC Biosearch Technologies | gfp smFISH probe #29 |
| gaccatgtggtcacgcttc | LGC Biosearch Technologies | gfp smFISH probe #30 |
| cggcggtgacgaactcgag | LGC Biosearch Technologies | gfp smFISH probe #31 |
| Chemicals, enzymes, and other reagents | ||
| 4% Paraformaldehyde | Sigma-Aldrich | #441244 |
| Deionized formamide | Thermo Fisher Scientific | #AM9342 |
| Dextran sulfate | Sigma-Aldrich | #D4911 |
| E. coli tRNA | Roche | #10109550001 |
| Vanadyl ribonucleoside complex | NEB BioLabs | #S1402 |
| Software | ||
| WormBase | https://wormbase.org/ Harris et al, 2020; Sternberg et al, 2024; Davis et al, 2022 | |
| CRISPOR | http://crispor.tefor.net Haeussler et al, 2016 | |
| Other | ||
| THUNDER Imager inverted widefield microscope | Leica | |
| BC43 spinning disk confocal microscope | Andor | |
| ChemiDoc XRS+ System | Bio-Rad | |