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. 2025 Aug 22;15:30881. doi: 10.1038/s41598-025-04308-6

Table 1.

The interaction impacts of the soil application of biochar × foliar-spraying of chitosan under varying soil moisture levels on the biochemical, phytochemical, and yield characteristics of yarrow during two years of study.

Irrigation Biochar (kg/m2 soil) Chitosan (g/L) Characteristics
Proline (μmol/g wet weight) Peroxidase (μmol/mg protein/min) Ascorbate peroxidase (μmol/mg protein/min) Catalase (μmol/mg protein/min) DPPH antioxidant capacity (%) Biological yield (kg/ha) Dry flower weight (kg/ha) Essential oil content (% v/w) Essential oil yield (L/ha)
Irrigation (80–85% F.C.) 0 0 14.4 k¶ 5.45 g 30.75 h 5.96 h 30.75 h 7714.4c 1161.6c 0.29i 3.52d
2.5 19.08i 5.93f. 32.56 h 7.31f. 32.56 h 8615.1b 1259.4b 0.31 h 3.97c
2 0 16.66j 5.59 g 31.19 h 6.7 g 31.19 h 8899.2b 1296.4ab 0.32 g 4.2b
2.5 21.45 h 6.3e 35.78 g 7.61ef 35.78 g 9197.7a 1323.3a 0.33 fg 4.39a
Irrigation (60–65% F.C.) 0 0 23.72 g 6.32e 38.59f. 7.95e 38.59f. 5660.7 g 805.4e 0.34f. 2.47 g
2.5 26.5f. 6.87d 44.06e 8.97d 44.06e 6602.7e 921.5d 0.35e 3.25e
2 0 25.21 fg 6.7d 40.26f. 8.67d 40.26f. 6050.6f. 841e 0.35e 2.99f.
2.5 32.8e 7.15c 46.75d 9.47c 46.75d 7182.6d 948.3d 0.38d 3.64d
Irrigation (40–45% F.C.) 0 0 37.11d 7.45b 53.66c 9.81c 53.66c 4135.9j 609.2 h 0.40c 2.46 h
2.5 46.92b 7.85a 61.75b 10.71b 61.75b 4652.9i 677.7 g 0.42b 2.79 g
2 0 40.28d 7.82a 60b 10.65b 60b 4458.2ij 648.6 g 0.43b 2.68 g
2.5 49.07a 7.72a 65.38a 12.51a 65.38a 5191.6 h 745.9f. 0.44a 3.29e
P-value 0.000** 0.01* 0.000** 0.000** 0.01* 0.03* 0.04* 0.00** 0.01*

¶ Different letters within a column indicate significant difference at p < 0.05 according to Duncan’s test.

*: significant at the 5% level and **: significant at the 1% level.