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. 2025 Aug 22;15:30881. doi: 10.1038/s41598-025-04308-6

Table 2.

The interaction impacts of the soil application of biochar × foliar-spraying of chitosan under varying soil moisture levels on the chemical compositions of the yarrow essential oil during two years of study.

Row Compositions RI Experimental treatments p-Value
Irrigation at 80–85% F.C Irrigation at 60–65% F.C Irrigation at 40–45% F.C
Non-Biochar Biochar Non-Biochar Biochar Non-Biochar Biochar
Non-Chitosan Chitosan Non-Chitosan Chitosan Non-Chitosan Chitosan Non-Chitosan Chitosan Non-Chitosan Chitosan Non-Chitosan Chitosan
1 α-Pinene 939 9.07j ¶ 9.63hi 9.92gh 9.39ij 10.05fg 10.88e 10.31f. 11.29d 12.05c 12.87b 13.13ab 13.39a 0.01*
2 Camphene 954 0.66h 0.85g 1.00f. 1.08ef 1.34d 1.17e 1.42d 1.55c 1.63bc 1.66bc 1.75b 2.19a 0.01*
3 Sabinene 975 1.73j 1.84i 1.94h 0.98k 2.34d 2.42c 2.70a 2.57b 2.07g 2.21ef 2.16f. 2.29de 0.000**
4 β-Pinene 979 1.44j 1.69i 1.63i 1.83h 2.40g 2.55ef 2.51f. 2.61e 2.73d 2.94c 3.22b 3.60a 0.000**
5 β-Myrcene 985 3.58i 3.82h 3.98gh 3.89h 4.09fg 4.23f. 4.27f. 4.60e 4.83d 5.03c 5.20b 5.73a 0.02*
6 α-Phellandrene 1003 2.03g 2.15f. 2.07g 1.23h 2.25e 2.38d 2.33d 2.44c 2.55b 2.61a 2.60ab 2.56ab 0.03*
7 p-Cymene 1029 0.28g 0.33f. 0.29g 0.36e 1.37c 1.42b 1.40b 0.44d 1.50a 1.51a 1.51a 1.49a 0.000**
Sum of hydrocarbon monoterpenes 18.79 20.31 20.83 18.76 22.84 25.05 24.94 25.50 27.36 28.83 29.57 31.10
8 1,8-cineole 1031 9.33h 10.22f. 10.40f. 9.84g 12.79d 13.25c 13.40c 11.55e 14.78b 15.12ab 15.29a 15.34a 0.01*
9 Pinocarveol < trans- >  1139 0.86h 0.90h 0.87h 0.94g 2.24b 2.41a 2.43a 1.52f. 2.00e 2.15c 2.10d 2.23b 0.000**
10 Verbenol < trans- >  1144 0.13i 0.17h 0.20g 0.22g 1.24e 1.27d 1.29d 0.31f. 1.33c 1.36b 1.34bc 1.45a 0.000**
11 Camphor 1146 2.90h 3.22g 3.08g 3.55f. 5.51c 5.99b 6.17ab 6.30a 3.83e 4.38d 4.00e 4.51d 0.000**
12 Borneol 1169 5.50gh 5.78g 5.57g 5.13h 6.33f. 8.78e 9.38d 6.40f. 10.52c 10.88c 12.71b 14.79a 0.000**
13 Terpinen-4-ol 1177 1.87j 2.04i 1.95ij 2.19h 2.37g 2.90e 2.74f. 3.15d 3.21d 3.79c 3.96b 4.18a 0.000**
14 Bornyl acetate 1285 2.13k 2.72i 2.59j 2.83h 3.74g 4.97d 4.56e 4.31f. 5.22c 5.35b 5.33b 7.02a 0.000**
Sum of oxygenated monoterpenes 22.72 25.05 24.66 24.71 34.22 39.57 39.97 33.57 40.89 43.03 44.73 49.52
Sum of hydrocarbon and oxygenated monoterpenes 41.51 45.36 45.49 43.46 58.06 64.62 64.91 59.04 68.25 71.86 74.3 80.77
15 Longifolene 1400 0.73b 0.78ab 0.86a 0.93a 0.59c 0.71b 0.65bc 0.67bc 0.42d 0.48d 0.53cd 0.56c 0.04*
16 Germacrene-D 1479 1.28a 1.34a 1.33a 1.29a 0.98b 1.10b 1.06b 1.17ab 0.75c 0.87bc 0.79c 0.96b 0.02*
17 α-Curcumene 1481 2.63b 2.82b 2.68b 3.47a 1.20d 0.47e 1.70d 0.81d 1.94c 1.01d 2.33bc 1.98c 0.000**
18 β-Bisabolene 1509 11.68b 12.05ab 12.96a 13.12a 9.98bc 9.60bc 8.80c 10.89b 7.85c 8.19c 5.70d 1.37e 0.001**
19 delta-Cadinene 1521 1.68a 0.81bc 1.05b 1.26b 1.20b 0.60 1.39ab 1.47ab 0.69c 0.87bc 1.03b 1.11b 0.02*
20 α-Calacorene 1546 1.87b 1.99ab 2.09ab 2.56a 1.46bc 0.73cd 0.57d 0.82c 0.92c 0.54d 1.11c 1.36bc 0.01*
21 α-Bisabolene < cis- >  1548 1.85a 1.96a 2.00a 2.04a 1.44b 1.52b 1.56b 1.49b 1.58b 1.69ab 1.61ab 1.75ab 0.02*
Sum of hydrocarbon sesquiterpenes 21.72 22.75 23.97 25.65 16.84 17.73 16.72 19.32 14.15 15.12 13.11 9.08
22 Nerolidol 1538 7.86b 8.45a 8.21ab 8.44a 6.16d 5.76de 5.48e 6.9c 4.72f. 4.51f. 3.62g 1.00h 0.000**
23 Caryophyllene oxide 1579 11.68c 12.05b 12.36ab 13.11a 9.97e 9.59f. 8.79g 10.88d 7.85i 8.18h 5.69j 1.36k 0.000**
24 Guaiol 1595 5.02a 5.22a 5.35a 5.57a 2.24b 2.71b 2.93b 3.82b 1.04c 1.25c 0.67d 2.07b 0.000**
25 Spathulenol 1635 1.38a 1.52a 1.51a 1.46a 1.14b 1.28b 0.24e 0.35e 0.85d 1.04c 0.96c 1.09c 0.02*
26 Chamazulene 1723 4.08b 5.16a 4.43b 5.39a 2.61d 2.91cd 2.77d 3.61c 1.58e 1.83e 2.10d 2.36 0.000**
Sum of oxygenated sesquiterpenes 30.02 32.40 31.86 33.98 22.12 22.25 21.21 26.56 15.77 16.46 14.04 7.88
Total 93.24 99.58 99.86 99.95 96.02 99.84 99.76 99.86 98.17 99.87 99.48 97.58

¶ Different letters within a row indicate significant difference at p < 0.05 according to Duncan’s test.

*: Significant at the five percent level and **: Significant at the one percent level.