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. 2025 Aug 22;15:30902. doi: 10.1038/s41598-025-15614-4

Table 2.

MS/MS parameters of the method.

Parameter Caffeine (CAF) Ibuprofen (IBU) Carbamazepine (CBM )
Ionization Mode HESI (positive) HESI (negative) HESI (positive)
MRM Transitions (m/z) 195-69 205-159 237-165
195-110 205-161 237-179
195-138 205-178 237-194
Collision Energy (V) 28.56 5.43 47.11
23.98 5.43 37.37
19.68 8.15 20.4
Dwell Time (ms) 98.6 118.5 98.6
Aux Gas (Arb) 15 15 15
Sheath Gas (Arb) 50 50 50
Ion Tranfer Tube Temp. (oC) 380 380 380
Vaporizer Temp (oC) 350 350 350
SheathGasFlow 50 50 50
Capillary (V) 3500 3500 3500
CID Gas (mTorr) 1.5 1.5 1.5