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. 2025 Jun 9;12(9):uhaf151. doi: 10.1093/hr/uhaf151

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© The Author(s) 2025. Published by Oxford University Press on behalf of Nanjing Agricultural University.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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The activity of pBnaA10/C09.TFL1^IDTI-A with the GT1-motif or P-box elements is significantly reduced, reaching comparable levels to those of pBnaA10/C09.TFL1^DTI-A. (a) Comparison of LUC/REN activity between constructs driven by pBnaA10/C09.TFL1^IDTI-A and pBnaA10/C09.TFL1^DTI-A. (b) Schematic diagram of functional element distribution in key differences (segment A) of the BnaA10/C09.TFL1 promoter. (c) Quantification of LUC/REN activity after single element site mutation in pBnaA10/C09.TFL1^IDTI-A compared to that of pBnaA10/C09.TFL1^IDTI-A and pBnaA10/C09.TFL1^DTI-A. Data correspond to the mean ± SD of three biological replicates. Different letters indicate significant differences by Tukey’s multiple comparison test with P < 0.01. Asterisks indicate significant differences (Student’s t-test, ^**P < 0.01).