Table 1.

Quantification of MT binding affinity for wild-type and mutated augmin N-clamp complexes from different species as determined using tubulin co-sedimentation assays

Sample	Kd [μM]	SD of Kd	R2	Number of experiments
H. sapienswild-type	0.8	0.4	0.94	5
X. laeviswild-type	0.6	0.4	0.91	3
A. thalianawild-type	0.01	0.09	0.89	3
D. melanogasterwild-type	0.4	0.3	0.90	4
D. melanogasterMsd5-GST	0.04	0.01	0.90	3
H. sapiensHAUS8-ΔNT	–	–	–	3
X. laevisHAUS8-ΔNT	2.2	2.1	0.69	3
A. thalianaAUG8-ΔNT	2.1	1.5	0.89	3
D. melanogasterDgt4- ΔNT	2.9	0.5	0.99	3
H. sapiensHAUS6-4A-mut	5.6	1.8	0.98	3
D. melanogasterDgt6-3A-mut	25.5	31.9	0.94	3
D. melanogasterMsd5- Δ81–93	1.0	1.0	0.61	3

Apparent K_d values, standard deviation (SD) and R² of the fitted curve (see Methods, Figs. 1–3) are shown. Dgt4/AUG8 and Dgt6 are the D. melanogaster/A. thaliana orthologs of HAUS8 and HAUS6, respectively. Number of experiments is given. No binding was observed for H. sapiens^HAUS8-ΔNT(-).