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H2 concentration (open squares) and methane production (solid squares) by M. thermoautotrophicus TM in a 1.3-liter serum bottle containing 500 ml of medium. Arrows indicate the sampling points for RNA preparation; 28.3, 37.6, and 46.8 mg of dry cells (arrows 1, 2, and 3, respectively) were harvested and used for total RNA extraction and subsequent Northern analysis. Error bars indicate standard deviations.
