FIG. 3.
Time course of acetate degradation (solid circles), methane production (solid squares), and H2 concentration (open squares) in a syntrophic acetate-oxidizing coculture (M. thermoautotrophicus TM plus T. phaeum PB) grown with 80 mM acetate in a 1.3-liter serum bottle containing 500 ml of medium. Arrows indicate the sampling points for RNA preparation; 13.1 and 65.7 mg of dry cells (arrows 1 and 2, respectively) were harvested and used for total RNA extraction and subsequent Northern analysis. Error bars indicate standard deviations.