FIG. 5.

Comparison of protein expression patterns determined for M. thermoautotrophicus TM in pure culture (A) and syntrophic coculture (B) by 2-DE analysis. Gels were stained with Coomassie brilliant blue R-350. The molecular masses of the markers are shown on the right. The pH gradient on the gels was from 10 (left) to 3 (right). The arrows indicate the proteins for which N-terminal amino acid sequences were determined. Each spot was identified as follows: 1, methyl-H₄MPT:HS-CoM methyltransferase subunit H (MtrH); 2, F₄₂₀-dependent N⁵,N¹⁰-methylene-H₄MPT reductase (Mer); 3, MRI γ; 4, MRI β; 5 and 9, MRI α; 6, MRII γ; 7, methyl viologen-reducing hydrogenase gamma subunit (MVH γ); 8, heterodisulfide reductase chain B (HDR); 10, MRII α.