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Growth of the parental and xyl3::URA3 disruptant strains on various carbon sources. (A) P. stipitis FPL-UC7 (ura3); (B) P. stipitis FPL-YS30 (xyl3::URA3). YP medium (10 g of yeast extract, 20 g of Bacto peptone, and 20 g of agar per liter) with 20 g of each carbon source per liter was used, and cells were serially diluted. Approximately 104 cells were spotted in the undiluted (top) droplet.
