TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Source or reference |
|---|---|---|
| Strains | ||
| P. stipitis CBS 6054 | Wild type | CBSa |
| P. stipitis UC7 | ura3-2 | 27 |
| P. stipitis YS30 | Isogenic to P. stipitis UC7 except xyl3::URA3 | This study |
| S. cerevisiae 679 | MATα trp1-289 | M. Culbertson |
| S. cerevisiae 679C | S. cerevisiae 679(pRS424) | This study |
| S. cerevisiae 679[pYS31N] | S. cerevisiae 679(pYS31N) | This study |
| S. cerevisiae 679[pYS32N] | S. cerevisiae 679(pYS32N) | This study |
| Plasmids | ||
| pRS314 | TRP1, CEN/ARS | 42 |
| pRS424 | TRP1, 2μm origin | 6 |
| pX3 | XYL3 in pBluescript KS(+) | This study |
| pYS03 | XYL3 disruption cassette containing xyl3::URA3 | This study |
| pYS31N | XYL3 in pRS314 | This study |
| pYS32N | XYL3 in pRS424 | This study |
a
CBS, Centraalbureau voor Schimmelcultures (Delft, The Netherlands).