Figure 2.

Quantification of Serially Diluted HBV Amplicon by Qualitative PCR and Digital PCR. The DNA amplicon was serially diluted from 10⁸ copies/μL to 10⁰ copies/μL. (a) qPCR amplification plot at 10-fold serial diluted HBV amplicon levels, (B) qPCR standard curve from a 10-fold dilution series of HBV amplicons, the slope of the fitted line is -0.349 and R² = 0.9936, (c) digital PCR analysis plot at low levels of HBV amplicons, (d) real-time PCR amplification plot at low levels of HBV amplicons.