Table 1.

Sequences of Primers for Nested-PCR assay

Gene	Kí hiệu	Trình tự mồi (5’-3’)	XoC	L
DAPK [25]	DAPK_S1-F*	GTTTGTAGGGTTTTTATTGGT	50	220
	DAPK_S1-R*	AAAACACAACTAAAAAATAAAT
	DAPK-M-F	GATAGTCGGATCGAGTTAACGTC	50	102
	DAPK-M-R	CAAATCCCTCCCAAACGCCGA
	DAPK-U-F	GGAGGATAGTTGGATTGAGTTAATGTT	50	108
	DAPK-U-R	CACAAATCCCTCCCAAACACCAA
p14 ARF [14]	P14-S1_F*	GGTGCGTGGGTTTTAGTTTGTA	52	256
	P14-S1_R*	AAC ATC AAC ACG AAA ACC ACA A
	P14-MF	GTGTTAAAGGGCGGCGTAGC	59	122
	P14-MR	AAAACCCTCACTCGCGACGA
	P14-UF	TTTTTGGTGTTAAAGGGTGGTGTAGT	59	132
	P14-UR	CACAAAAACCCTCACTCACAACAA
p15 INK4α [14]	P15_S1-F*	GGTTTAGTTGAAAACGGAATTT	50	236
	P15_S1-R*	TCTCTCCTTCCTAAAAAACCTAAA
	P15-MF	GCGTTCGTATTTTGCGGTT	55	148
	P15-MR	CGTACAATAACCGAACGACCGA
	P15-UF	TGTGATGTGTTTGTATTTTGTGGTT	55	154
	P15-UR	CCATACAATAACCAAACAACCAA
p16 INK4α [32]	P16_S1-F	GGAGGAAGAAAGAGGAG	50	277
	P16_S1-R	CTACAAACCCTCTACCCA
	P16-M-F	TTATTAGAGGGTGGGGCGGATCGC	55	150
	P16-M-R	GACCCCGAACCGCGACCGTAA
	P16-U-F	TTATTAGAGGGTGGGGTGGATTGT	58	151
	P16-U-R	CAACCCCAAACCACAACCATA

M, Methyl; U, Unmethyl; F, Forward primer; R, Reverse primer, X^oC, annealing temperature