Skip to main content
. 2025 Jul 24;15(16):8049–8067. doi: 10.7150/thno.108869

Figure 2.

Figure 2

LIG induces HSC microfilament rearrangement. (A) The heatmap of F-actin polymerization and depolymerization genes. (B-C) IF staining of F-actin in primary hepatocyte (B) and HSC (C), accompanied by the quantification results. (D) F-actin and G-actin extracted from LX-2 cells treated with TGF-β or LIG for 24 h are subjected to western blot with actin and α-SMA. (E) The diagram of LIG and G-actin molecular docking. (F) G-action polymerization assay by actin polymerization kit. (G) The diagram illustrates how LIG inhibits G-actin polymerization. (H) F-actin and G-actin that extracted from LX-2 cells treated with TGF-β, JAS or LIG for 24 h are isolated and detected with WB. (I) IF co-staining of F-actin (red) and DAPI (cyan). Statistical significance: ## P < 0.01, compared with the TGF-β group; **P < 0.01.