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. 2025 Jul 24;15(16):8202–8221. doi: 10.7150/thno.113382

Figure 4.

Figure 4

ARHGEF12 depletion decreases cell migration capacity in GC. (A) Western blotting (WB) of ARHGEF12 expression in gastric mucosal epithelial cell line GES and six gastric cancer cell lines. (B) WB of ARHGEF12 knockdown (KD) in AGS and MKN45 cells. A control shRNA (shCtrl) and two different ARHGEF12 shRNAs (shARH12 #1 and shARH12 #2) were used. (C-E) Effects of ARHGEF12 KD on AGS and MKN45 cells migration (C), invasion (D) and colony formation (E). (F) Representative immunofluorescence (IF) images of AGS and MKN45 cells with or without ARHGEF12 KD. Red: F-actin, Blue: DAPI. Scale bar, 10 µm. (G) Quantification of the number of filopodia. Data shown are from 30 cells counted per condition. (H-I) Cell migration (H) and cell invasion (I) analysis of AGS cells (high ARHGEF12 expression) or MGC823 cells (low ARHGEF12 expression). Cells were pre-treated with or without 50 µM Y16 for 48 h and then subjected to cell migration and cell invasion assays. (J) Quantification of the number of filopodia. Data shown are from 30 cells counted per condition. Data are representative of three independent experiments with similar results. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, by two-tailed Student's t-test or one-way ANOVA analysis.