Skip to main content
. 2025 Jul 24;15(16):8202–8221. doi: 10.7150/thno.113382

Figure 6.

Figure 6

ARHGEF12 E620K mutation activates Rap1 signaling pathway and upregulates integrin gene expression. (A) Heatmap of mRNA-Seq analysis of differentially expressed genes regulated by ARHGEF12 mutant E620K (fold change >2, P <0.05). (B-C) GO enrichment analysis of downregulated genes (G1) by ARHGEF12 KO (B) or upregulated genes (G2) by the E620K mutant (C). (D) Gene set enrichment analysis (GSEA) showed enrichment of Rap1 signaling pathway and integrin-related gene signature in E620K mutated cells. NES, normal enrichment score. (E) WB of effects of ARHGEF12 E620K mutation on Rap1 signaling activation (Rap1A-GTP, Talin, and p-ERK) in AGS cells. (F-G) qRT-PCR analysis of relative mRNA levels of integrin α (F) and β (G) family members regulated by the E620K mutant in AGS cells. Data are representative of three independent experiments with similar results. Data were presented as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001, by two-tailed Student's t-test.