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. 2025 Jul 25;15(16):8259–8319. doi: 10.7150/thno.116153

Table 3.

Safety studies of UCNPs in rodents.

UCNPs Surface modification Size (nm) Zeta potential (mV) Animal Route Dosage Results Ref.
Ba2GdF7:Yb,Er PEG 24±5 25.73 Nude mice (25 g) Tail vein injection 2-4 mg/mL (0.2 mL) Major organ pathological sections normal; Hemolysis assay confirmed no blood cell damage 163
BaYbF5:Tm PAA 14.7±3.5 -19.8 Kunming mice Oral 100 mg/mL (0.4 mL) Major organ pathological sections normal; Female mice litter size unchanged 370
Anus 20 mg/mL (0.8 mL) Rectal mucosa epithelium intact, no ulcers or inflammatory cell infiltration;
Wistar
rats
Oral 20 mg/mL (5 mL) Blood concentrations of Ba2+, Yb3+ < 0.1 ng/mL
Anus 20 mg/mL (5 mL) In vitro hemolysis rate < 0.5%
Lu2O3:Gd,Yb,Er PEG 85-130 -0.65 Kunming mice Tail vein injection 100 mg/kg Body weight stable; normal diet, activity, and hair; major organ pathological sections normal; Hematological and biochemical parameters normal; Female mice litter size unchanged 372
NaGdF4:Tm,Er,Yb Azelaic acid (carboxylic acid) 25-60 - Kunming mice (20 g) Tail vein injection 1.5 mg/kg No significant weight loss, abnormal behavior, or reproductive changes 374
NaLuF4:Yb,Er Polypyrrole; PEG; DNA; DOX 70 4.6 BALB/c nude mice (16-18 g) Intravenous injection 9.4 mg/kg Major organ pathological sections normal; Hematological and biochemical parameters normal 379
NaLuF4:153Sm,Yb,Tm 6-aminohexanoic acid 25-30 - Kunming mice (20 g) Tail vein injection 20 mg/kg Major organ pathological sections normal; Hematological and biochemical parameters normal 385
Gd2O3:Yb,Er PEG 90-150 - Kunming mice Tail vein injection 10 mg/kg Body weight gain patterns normal; Mild inflammation in liver/spleen tissues; Hematological and biochemical parameters normal 66
NaLuF4:Yb,Er Polyaniline NPs; Pluronic F127 120 0 NU/NU nude mice Intravenous injection 2 mg/mL (0.1 mL) Major organ pathological sections normal; Biochemical and renal parameters normal 386

PEG: polyethylene glycol; PAA: polyacrylic acid; PEI: polyethylene imine; FA: folic acid; DOX: doxorubicin; NPs: nanoparticles; Pluronic F127: poly (ethylene glycol)-block-poly (propylene glycol)-block-poly (ethylene glycol).