Figure 2.

Tan I improves the neuronal structure and neuronal activity in the mPFC of CUS mice. (A) The representative transmission electron microscopy of different groups of mice (n = 5). Scale bar = 0.5 µm. Orange arrows show synapses. (B) The number of synapses per 20 μm² was calculated (n = 5). (C) Golgi-Cox staining shows representative images of the dendritic spines of pyramidal neurons from different groups of mice. Scale bar = 5 μm. (D) Quantification of dendritic spine density of pyramidal neurons in different groups of mice (n = 5). (E) The levels of PSD95 and SYP in the mPFC were analyzed. (F-G) Relative quantification of PSD95 and SYP protein levels of CUS mice after Tan I treatment (n = 4). (H) Representative images of PSD95 (green) and SYP (red) in different groups of mice. Scale bar = 10 μm. Colocalized puncta were indicated by circles. (I) Quantification of the colocalization of SYP and PSD95 in different groups of mice (n = 5). (J-K) Representative images (J) and quantitative analysis (K) of NeuN⁺ (red) and c-Fos⁺ (green) in the mPFC of CUS mice after Tan I treatment (n = 5). Scale bar = 40 μm. (L-M) Representative images (L) and quantitative analysis (M) of c-Fos⁺ (green) and CaMKIIα⁺ (red) in the mPFC of CUS mice after Tan I treatment (n = 5). Scale bar = 40 μm. ^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001 versus vehicle control group; ^#P < 0.05, ^##P < 0.01, ^###P < 0.001 versus vehicle CUS group; ns, not significant. One-way ANOVA with Tukey's post hoc test. All data are expressed as mean ± SEM.