TABLE 3.
Species | Gut section | Expta | Temp (°C) | Turnover constantb (min−1) | Mean pool sizec (μmol ml−1) | Acetate turnoverd (nmol ml−1 min−1) |
---|---|---|---|---|---|---|
K. sydneyanus | IV | 1 | 19 | 0.0051 | 55.2 ± 7.6 | 281 |
2 | 22.5 | 0.015 | 29.2 ± 4.1 | 438 | ||
3 | 16 | 0.02 | 32.2 ± 4.6 | 655 | ||
4 | 18.5 | 0.019 | 38.9 ± 8.6 | 747 | ||
5 | 18.5 | 0.027 | 26.9 ± 7.6 | 724 | ||
V | 1 | 19 | 0.0145 | 42.7 ± 3.9 | 619 | |
2 | 22.5 | 0.0063 | 44.3 ± 7.4 | 274 | ||
3 | 16 | 0.0162 | 27.6 ± 1.5 | 450 | ||
4 | 18.5 | 0.0107 | 42.0 ± 4.2 | 452 | ||
O. pullus | IV | 1 | 20 | 0.04 | 18.6 ± 3.8 | 749 |
2 | 20 | 0.036 | 14.5 ± 3.4 | 525 | ||
3 | 16 | 0.028 | 13.3 ± 2.3 | 381 | ||
V | 1 | 20 | 0.021 | 5.9 ± 1.1 | 130 | |
2 | 20 | 0.046 | 10.8 ± 2.5 | 497 | ||
3 | 18 | 0.025 | 19.7 ± 8.5 | 492 | ||
A. arctidens | IV | 1 | 19 | 0.0256 | 10.1 ± 4.2 | 259 |
2 | 16 | 0.0246 | 14.9 ± 7.6 | 365 | ||
V | 1 | 22 | 0.0064 | 18.1 ± 4.9 | 116 | |
2 | 19 | 0.0069 | 20.3 ± 4.9 | 140 | ||
3 | 16 | 0.0126 | 10.6 ± 4.8 | 134 |
In each experiment samples were removed from the gut section in duplicate at various intervals over 120 min (n = 5 and 4 for sections IV and V of K. sydneyanus and 3 for the same gut sections in O. pullus and A. arctidens, respectively).
Determined as the slope of plots of log specific activity [2-14C]acetate versus time (see Fig. 2) × 2.303.
Means are for samples taken in duplicate over the time course of the experiment (120 min) ± one standard deviation.
Determined as the pool size versus turnover rate constant.