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. 2025 Jul 25;15(16):8488–8508. doi: 10.7150/thno.114534

Figure 1.

Figure 1

Synthesis and characterization of GB4-BPL. (A) Schematic diagram of BP-lipid nanoparticles screening for efficient gene delivery to HEK-293T cells. Fluorescence intensity expression in HEK-293T cells after treatment with the BP lipids encapsulating EGFP plasmid. 5000 cells were incubated with lipid nanoparticles at a EGFP plasmid dose of 10 ng/well (n = 3, mean ± SD). (B) Schematic illustration of synthesizing GB4-BPL. (C) TEM images of GB4-BPL; scale bars = 100 nm. (D-F) Transfection assessments of GB4-BPL@pEGFP complexes on HEK-293T cells at different mass ratios. D) Images of fluorescence microscope; scale bars = 50 μm. E, F) Fluorescence intensity results of fluorescence microplate reader at 24, 48 h (n = 4, mean ± SD), ns: no significance, one-way ANOVA. (G) Transfection assessments of GB4-BPL@pEGFP complexes on RM-1 EnzR cells at 48 h, Lipo8000@pEGFP as control (n = 3, mean ± SD), ns: no significance, one-way ANOVA. (H) Cytotoxicity of GB4-BPL at different concentrations in NIH3T3 cells, Lipo8000 was used as control, GB4-BPL: 0~3 mg/mL (n = 6, mean ± SD, one-way ANOVA).