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. 2025 Jul 25;15(16):8488–8508. doi: 10.7150/thno.114534

Figure 2.

Figure 2

The cellular uptake of GB4-BPL in vitro. (A, B) Cellular uptake of GB4-BPL detected by flow cytometry in RM-1 EnzR cells and PTEN.CaP8 EnzR cells at 1 h and 4 h, siFAM: 20 nM. (n = 3, mean ± SD), *p < 0.05, **p < 0.01, ***p < 0.001, one-way ANOVA. (C) Images of intracellular distribution. Cy3-siRNA: 20 nM, scale bars = 20 μm. (D) Images of lysosome escape. siFAM: 20 nM, Lysotracker red: 50 nM, scale bars = 20 μm. (E) 3D tumor spheroid model. DiO-stained RM-1 EnzR cells and DiD-stained MC3T3 cells were seeded in the 96-well plates at the ratio of 1:1. Blue fluorescence: DAPI, green fluorescence: DiO, red fluorescence: DiD, scale bars = 100 μm. (F) The images of in vitro tumor spheroids penetration of each group were acquired at 10× objective magnification using Z-stack imaging, scale bars = 100 μm.