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. 2025 Jul 25;15(16):8488–8508. doi: 10.7150/thno.114534

Figure 3.

Figure 3

In vitro anti-proliferation ability tests. (A-F) The cytotoxicity results of each group in A, B) RM-1 EnzR cells for 48 h (n = 6), C, D) PTEN.CaP8 EnzR cells for 48 h (n = 6) and E, F) RM-1 EnzR cells for 48 h (n = 4). (G, H) 22RV1 cells for 48 h (n = 4) (siCXCR2: 0~40 nM, pPTEN: 0~3 μg/mL, Enz: 0~100 μg/mL). (I) The representative results of apoptosis of RM-1 EnzR cells treated with each group for 48 h (siCXCR2: 8.3 nM, pPTEN: 1.156 μg/mL). (J) Statistical results of cell apoptosis in each group (n = 3). (K) The representative results of apoptosis of RM-1 EnzR cells treated with each group for 48 h (Enz: 30 µg/mL, siCXCR2: 8.3 nM, pPTEN: 1.156 μg/mL). (L) Statistical results of cell apoptosis in each group (n = 3). (M) RM-1 EnzR cells were incubated with each group for 24 h (anti-migration assay) and 48 h (anti-invasion assay, with matrix gel to mimic tumor extracellular matrix), Enz: 14.16 μg/mL, siCXCR2: 2.1755 nM, pPTEN: 0.354 μg/mL. (scale bars = 50 μm). (N, O) Statistical results of anti-migration assay and anti-invasion assay in each group in each group (n = 3). (P-S) The RT-qPCR results of the expression of CXCR2 and PTEN mRNA in P, Q) RM-1 EnzR cells and R, S) PTEN.CaP8 EnzR cells (n = 3). Data are represented as means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, ns: no significance, one-way ANOVA.