FIG. 7.
Analysis of protease II by Western blotting. X. nematophila growth media collected at different times and purified protease II were transferred to nitrocellulose membranes. Proteins were detected by Western blotting using antibodies raised against Prot.II-29 peptide as primary antibody. Lanes: 1, 18 h of growth in medium; 2, 24 h of growth in medium; 3, purified fraction.
