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Purification of X. nematophila protease II
| Sample analyzed | Total vol (ml) | Total protein (mg) | Total activity (U)a | Sp act (U/mg) | Yield (%) |
|---|---|---|---|---|---|
| Concentrated supernatant | 15 | 18.35 | 97 | 5 | 100 |
| DEAE Sepharose | 12 | 0.62 | 79 | 126 | 81 |
| HiTrap Q Sepharose | 8 | 0.27 | 37 | 135 | 38 |
| Mono Q | 5 | 0.11 | 19 | 176 | 20 |
One unit of protease activity was defined as the amount of enzyme required to produce an absorbance change of 0.01 under the conditions of the assay.
