TABLE 3.

Inhibitor reactivity with protease II

Inhibitor	Inhibitor classa	Solvent	Inhibitor concn	% Activity remainingb
None				100
Antipain dihydrochloride	Serine	H2O	1 mM	79
Chymostatin	Serine	DMSOc	100 μM	55
Leupeptin	Ser/Cys	H2O	1 μM	116
Pefabloc SC	Serine	H2O	4 mM	7
Aprotinin	Serine	H2O	0.3 μM	103
E-64	Cysteine	50% Ethanol	5 μM	114
Pepstatin	Aspartic	Methanol	1 μM	94
Bestatin	Metallo	Methanol	130 μM	105
Phosphoramidon	Metallo	H2O	570 μM	96
EDTA	Metallo	H2O	1.3 mM	98
EDTA	Metallo	H2O	20 mM	57

^aInhibitor class refers to those proteases known to be susceptible to each compound tested.

^bEnzyme samples were incubated with an inhibitor for 30 min at 37°C and then the remaining activities in the sample were determined. Each value represents the mean of three experiments and the activity in the reaction mixture containing no inhibitor was considered 100% activity

^cDMSO, dimethyl sulfoxide.