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. 2025 Jul 28;21(11):4942–4960. doi: 10.7150/ijbs.112133

Figure 1.

Figure 1

HBx expression-upregulated MALAT1 is positively correlated with poor prognosis in HCC patients. A-C Three HCC gene expression datasets, including GSE6764 (n = 45), GSE62232 (n = 26), and GSE98269 (n = 6), were retrieved from the GEO database. The raw data were normalized, and the numbers of DEGs (P < 0.05 and |log2FC| ≥ 1.2) were 1043, 1219 and 3379, respectively, as shown in the heatmap and volcano plots. D Venn diagram showing the overlap between the DEGs of the three cohorts. E Heatmap of 10 HBx-related HCC-associated lncRNAs in HBx-Tg mice (n = 4) and WT mice (n = 4). F-G Relative expression of 7 candidate lncRNAs was detected via qRT-PCR in HBV/HBx-expressing HCC cells. H Relative expression of 7 candidate lncRNAs was detected via qRT-PCR in HBx-Tg mice livers. I Pan-cancer analysis of MALAT1 expression in the TCGA-LIHC cohort. J-K Quantification of MALAT1 expression in HBV-infected and uninfected HCC tissue and peritumor tissue samples. L GSEA of MALAT1 in the TCGA-LIHC cohort. NES, normalized enrichment score. M-N Paired tumor and adjacent peritumor liver tissues were collected from 12 patients with HBV-related HCC. M Relative expression of MALAT1 was detected by qRT-PCR. N Quantification of FISH results showing fluorescence intensity and percentage of MALAT1-positive area. O Kaplan-Meier analysis of the OS and DFS of patients with MALAT1, with or without hepatitis infection in the TCGA-LIHC cohort. HR, hazard ratio. *P < 0.05; **P < 0.01; ***P < 0.001. n.s., not significant.