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. 2025 Jul 28;21(11):4942–4960. doi: 10.7150/ijbs.112133

Figure 2.

Figure 2

MALAT1 promotes the proliferation and metastasis of HBV/HBx-expressing HCC cells in vitro. A-C HepG2.2.15 cells were transfected with ASO-MALAT1 (100 nM, for 24 h) to construct MALAT1-silence cells, while ASO-NC was used as a negative control (NC). RNA sequencing was performed for transcriptomic profiling analysis in cells. A Volcano plot illustrating DEGs. B GO enrichment analysis of DEGs. C KEGG enrichment analysis of DEGs. D-I HepG2.2.15 and HBx-expressing HepG2 cells were transfected with ASO-MALAT1 (100 nM, for 24 h), while ASO-NC was used as a control. D Colony formation assays were performed to determine the clonogenicity of the cells. The relative number of colonies is shown. E Cell proliferation was detected using EdU staining (green). Nuclei were counterstained with Hoechst 33342 (blue). Scale bar: 100 μm. F-G The levels of E-cadherin and Vimentin were detected by WB (F) and IF staining (G). Scale bar: 20 μm. H Cell migration was measured by wound healing assays. I The migration and invasion of the cells were examined via Transwell assays. *P < 0.05; **P < 0.01; ***P < 0.001. n.s., not significant.