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. 2025 Jul 24;21(11):4782–4797. doi: 10.7150/ijbs.105391

Figure 2.

Figure 2

M16 promotes CRC progression in vitro and in vivo. A-D. qRT-PCR and Western blotting were used to confirm M16 knockdown and overexpression in CRC cells. E. CCK-8 assay was used to evaluate the proliferation capacity of CRC cells with M16 knockdown or overexpression. F-G. Colony formation ability of CRC cells with M16 knockdown or overexpression was assessed using plate colony formation assays. H-K. Migration and invasion capabilities of CRC cells with M16 knockdown or overexpression were analyzed by Transwell assays. L. Xenograft tumors from SW480-shNC, SW480-shM16-1, SW480-shM16-2, SW480-vector, and SW480-OE-M16. M. Growth curve of xenograft tumors was monitored and plotted over a specified period. N. Xenograft tumor weights were measured after euthanizing the mice. O. IHC analysis of M16 and Ki67 expression in xenograft tumors. *p < 0.05, **p < 0.01, ***p < 0.001.