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. 2025 Jul 28;21(11):4908–4926. doi: 10.7150/ijbs.116639

Figure 3.

Figure 3

The LINC02802/miR-1976 axis regulates proliferation and migration in lung adenocarcinoma. (A) Bioinformatics analysis combined with RNA pull-down assays to identify miRNAs interacting with LINC02802. (B) RNA pull-down using a LINC02802-specific probe to validate its binding with miR-1976. (C) Dual-luciferase reporter assays confirming direct interaction between LINC02802 and miR-1976. (D-E) TCGA-based analysis of miR-1976 expression and its correlation with LINC02802 in LUAD. (F) ROC curve evaluating the diagnostic potential of miR-1976 in LUAD. (G) Kaplan-Meier survival analysis of miR-1976 in LUAD. (H-I) CCK-8 and colony formation assays demonstrating the regulatory effect of the LINC02802/miR-1976 axis on cell proliferation. (J-K) Transwell assays evaluating the impact of the LINC02802/miR-1976 axis on cell migration and invasion. (L-N) miR-1976 mimics suppress tumor formation in vivo by H1299 cells, Representative images (L), tumor volume (M), and tumor weight (N) are shown. (n = 3 mice per group) Data are presented as mean ± SD (bar plots). sh#1=LINC02802 shRNA#1, sh#2=LINC02802 shRNA#2, Ove=LINC02802 Overexpression. NC= negative control, *P < 0.05; **P < 0.01; ***P < 0.001; ns not significant.